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Choongwae Pharma Corporation
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Ratiopharm gmbh
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Elkins Sinn Corp
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Ratiopharm gmbh
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HELIOS Klinikum Aue
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LEO Pharma
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Becton Dickinson
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LEO Pharma
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Ayerst Laboratories
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Image Search Results
Journal: Scientific Reports
Article Title: Heparin Forms Polymers with Cell-free DNA Which Elongate Under Shear in Flowing Blood
doi: 10.1038/s41598-019-54818-3
Figure Lengend Snippet: Histones reverse the anticoagulant effects of heparin. ( a ) Citrated plasma was supplemented with 0.5 or 1 IU UFH, as well as a concentration range of exogenous histones. Clotting times (aPTT) experiments were subsequently determined in triplicate. Data represent means +/− SD. ( b ) Fibrin formation (green) in recalcified citrated whole blood on immobilised collagen under flow. Supplementation with 10 IU/mL UFH completely inhibits fibrin formation. This is reversed by either 500 µg/mL histones or 125 µg/mL protamine. Representative images were taken at the onset of fibrin formation (i.e. before the flow chamber becomes obstructed), in conditions where this occurs. Images were taken at t = 10 minutes (positive control), 20 minutes (UFH only), and 18 minutes (+histones, +protamine). Experiments were performed >4 times. Scale bars represent 20 µm.
Article Snippet: These included
Techniques: Clinical Proteomics, Concentration Assay, Coagulation, Positive Control
Journal: Scientific Reports
Article Title: Heparin Forms Polymers with Cell-free DNA Which Elongate Under Shear in Flowing Blood
doi: 10.1038/s41598-019-54818-3
Figure Lengend Snippet: Disruption of polymer formation. ( a ) by preincubation of 10 IU/mL heparin with protamine sulfate (125 µg/mL). ( b ) by preincubation of 10 IU/mL heparin with Heparinase I/III (5 U/mL) or DNAseI (10 µg/mL). Experiments were performed thrice, scale bars represent 10 µm.
Article Snippet: These included
Techniques: Disruption, Polymer
Journal: Scientific Reports
Article Title: Heparin Forms Polymers with Cell-free DNA Which Elongate Under Shear in Flowing Blood
doi: 10.1038/s41598-019-54818-3
Figure Lengend Snippet: Proposed mechanism for the formation of polymers between heparin and histones/DNA on the surface of platelet aggregates. Negatively charged heparin molecules (in orange) sequester circulating nucleosomes in plasma (in blue) by binding to protruding positively charged N-termini of histones. Under flow, these complexes attach to one or more currently unidentified platelet surface receptors, which we hypothesise to be glycoproteins.
Article Snippet: These included
Techniques: Clinical Proteomics, Binding Assay